Buffers ⋆ Morganville Scientific

TBS-T Buffer Tris Buffered Saline with Tween 20 Powdered Buffer Packs

morganvillesci — Fri, 27 Oct 2023 14:38:12 +0000

Accuris Quicksilver Tris Buffered Saline with Tween® 20 (TBS-T) powdered buffer packs: Add pre-measured pack to 1 L of dH2O and stir for fresh 1X TBS-T buffer.

Quick-dissolving (5 minutes or less). No pH adjustment necessary (pH 7.25-7.55).

TBS-T is a buffer used during Western blotting to make blocking buffer (with nonfat dry milk, for example), antibody dilution buffer and wash buffer.

Quicksilver buffers are available for use during nucleic acid electrophoresis, protein electrophoresis, Western blotting, ELISA and cell culture.

1X TBS-T solution contains 137 mM NaCl, 2.7 mM KCl, 50 mM Tris and 0.1% Tween® 20. Nuclease- and protease-free.

Powdered buffer packs come in a convenient, space-saving dispenser box, 100 pouches/pack.

Makes 100 L of 1 X TBS-T. Equivalent to 10 L of 10X TBS-T.

Accuris catalog # EB1203.

Cost saving equivalent replacement for VWR® TBS-T (Tris Buffered Saline, Tween-20) Buffer Powder Packs (cat # 76371-740).

Accuris TBS-T Buffer Tris Buffered Saline with Tween 20 Powdered Buffer Packs Product Insert.pdf

The post TBS-T Buffer Tris Buffered Saline with Tween 20 Powdered Buffer Packs appeared first on Morganville Scientific.

TBS Buffer Tris Buffered Saline Powdered Buffer Packs

morganvillesci — Thu, 26 Oct 2023 16:07:03 +0000

Accuris Quicksilver Tris Buffered Saline (TBS) powdered buffer packs: Add pre-measured pack to 1 L of dH2O and stir for fresh 1X TBS buffer.

Quick-dissolving (5 minutes or less). No pH adjustment necessary (pH 7.25-7.55).

TBS is a buffer that is used to make blocking buffer during Western blotting and ELISA where proteins are detected using fluorescence (TBS-T isn’t used during the blocking step when detecting proteins by fluorescence because Tween 20 auto-fluoresces). TBS-T can be used in subsequent steps. TBS is also used during immunohistochemistry.

Quicksilver buffers are available for use during nucleic acid electrophoresis, protein electrophoresis, Western blotting, ELISA and cell culture.

1X TBS solution contains 137 mM NaCl, 2.7 mM KCl and 50 mM Tris. Nuclease- and protease-free.

Powdered buffer packs come in a convenient, space-saving dispenser box, 100 pouches/pack.

Makes 100 L of 1 X TBS. Equivalent to 10 L of 10X TBS.

Accuris catalog # EB1202.

Cost saving equivalent replacement for VWR® TBS (TRIS Buffered Saline) Buffer Powder Packs (cat # 76371-738).

Accuris TBS Buffer Tris Buffered Saline Powdered Buffer Packs Product Insert.pdf

The post TBS Buffer Tris Buffered Saline Powdered Buffer Packs appeared first on Morganville Scientific.

PBS-T Buffer Phosphate Buffered Saline with Tween 20 Powdered Buffer Packs

morganvillesci — Fri, 20 Oct 2023 14:52:31 +0000

Accuris Quicksilver Phosphate Buffered Saline with Tween® 20 (PBS-T) powdered buffer packs: Add pre-measured pack to 1 L of dH2O and stir for fresh 1X PBS-T buffer.

Quick-dissolving (5 minutes or less). No pH adjustment necessary (pH 7.2-7.6).

PBS-T is a buffer used as a wash buffer during Western blotting and ELISA (Enzyme-linked Immunosorbent Assay).

Quicksilver buffers are available for use during nucleic acid electrophoresis, protein electrophoresis, Western blotting, ELISA and cell culture.

1X PBS-T solution contains 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate and 0.05% Tween® 20. Nuclease- and protease-free.

Powdered buffer packs come in a convenient, space-saving dispenser box, 100 pouches/pack.

Makes 100 L of 1 X PBS-T. Equivalent to 10 L of 10X PBS-T.

Accuris catalog # EB1201.

Cost saving equivalent replacement for VWR® PBS-T (Phosphate Buffered Saline – Tween-20) Buffer Powder Packs (cat # 76371-736).

Accuris PBS-T Buffer Phosphate Buffered Saline with Tween 20 Powdered Buffer Packs Product Insert.pdf

The post PBS-T Buffer Phosphate Buffered Saline with Tween 20 Powdered Buffer Packs appeared first on Morganville Scientific.

PBS Buffer Phosphate Buffered Saline Powdered Buffer Packs

morganvillesci — Thu, 19 Oct 2023 15:03:02 +0000

Accuris Quicksilver Phosphate Buffered Saline (PBS) powdered buffer packs: Add pre-measured pack to 1 L of dH2O and stir for fresh 1X PBS buffer.

Quick-dissolving (5 minutes or less). No pH adjustment necessary (pH 7.2-7.6).

PBS is a buffer that has a variety of uses. It is used during the manipulation of cells, as it maintains osmotic balance and pH for cells in solution. It is also used as a blocking buffer (along with nonfat dried milk) during Western blotting and a blocking buffer (along with Bovine Serum Albumin) during ELISA.

Quicksilver buffers are available for use during nucleic acid electrophoresis, protein electrophoresis, Western blotting, ELISA and cell culture.

1X PBS solution contains 137 mM NaCl, 2.7 mM KCl and 10 mM phosphate. Nuclease- and protease-free.

Powdered buffer packs come in a convenient, space-saving dispenser box, 100 pouches/pack.

Makes 100 L of 1 X PBS. Equivalent to 10 L of 10X PBS.

Accuris catalog # EB1200.

Cost saving equivalent replacement for VWR® PBS (Phosphate Buffered Saline) Buffer Powder Packs (cat # 76371-734).

Accuris PBS Buffer Phosphate Buffered Saline Powdered Buffer Packs Product Insert.pdf

The post PBS Buffer Phosphate Buffered Saline Powdered Buffer Packs appeared first on Morganville Scientific.

TGS Buffer Tris-Glycine-SDS Powdered Buffer Packs

morganvillesci — Mon, 16 Oct 2023 19:51:35 +0000

Accuris Quicksilver Tris-Glycine-SDS (TGS) powdered buffer packs: Add pre-measured pack to 1 L of dH2O and stir for fresh 1X TGS buffer.

Quick-dissolving (5 minutes or less). No pH adjustment necessary (pH 8.1-8.5).

TGS is used as a running buffer during SDS-PAGE of proteins using Tris-HCl polyacrylamide gels.

Quicksilver buffers are available for use during nucleic acid electrophoresis, protein electrophoresis, Western blotting, ELISA and cell culture.

1X TGS solution contains 25 mM Tris, 192 mM Glycine and 0.1% SDS. Nuclease- and protease-free.

Powdered buffer packs come in a convenient, space-saving dispenser box, 50 pouches/pack.

Makes 50 L of 1 X TGS. Equivalent to 5 L of 10X TGS.

Accuris catalog # EB1100.

Cost saving equivalent replacement for VWR® TRIS Glycine-SDS Buffer Powder Packs (cat # 76371-728).

Accuris TGS Buffer Tris-Glycine-SDS Powdered Buffer Packs Product Insert.pdf

The post TGS Buffer Tris-Glycine-SDS Powdered Buffer Packs appeared first on Morganville Scientific.

TAE Buffer Tris-Acetate-EDTA Powdered Buffer Packs

morganvillesci — Mon, 19 Oct 2020 20:38:41 +0000

Accuris Quicksilver Tris-Acetate-EDTA (TAE) powdered buffer packs: Add pre-measured pack to 1 L of dH2O and stir for fresh 1X TAE buffer.

Quick-dissolving (5 minutes or less). No pH adjustment necessary (pH 8.2-8.4).

TAE buffer is ideal for use during agarose gel electrophoresis of DNA.

Quicksilver buffers are available for use during nucleic acid electrophoresis, protein electrophoresis, Western blotting, ELISA and cell culture.

1X TAE solution contains 40 mM Tris, 20 mM acetic acid and 1 mM EDTA. Nuclease- and protease-free.

Powdered buffer packs come in a convenient, space-saving dispenser box, 100 pouches/pack.

Makes 100 L of 1 X TAE. Equivalent to 2 L of 50X TAE.

Accuris catalog # EB1000.

Cost saving equivalent replacement for VWR® TAE Buffer Powder Packs (cat # 76371-722).

Accuris TAE Buffer Tris-Acetate-EDTA Powdered Buffer Packs Insert.pdf

The post TAE Buffer Tris-Acetate-EDTA Powdered Buffer Packs appeared first on Morganville Scientific.