Use the Accuris large UV transilluminator to analyze bands of DNA that have been separated by agarose gel electrophoresis, and stained with ethidium bromide (EtBr), SYBR Green or Accuris SmartGlow, for genotyping purposes, for example.

The scratch-resistant ultraviolet filter glass provides 302 nm UV light (300-312 nm range).  A hinged, UV-protective acrylic lens allows the user to position the lens at an angle and view the illuminated gel safely while allowing access to the gel for band excision.

The large UV transilluminator allows the user to adjust the light intensity with a knob (i.e. variable adjustment), unlike the smaller model, which has only one UV intensity level (i.e. on and off).  Lowering the intensity of the UV light will help prevent DNA damage while the user works to excise the DNA band from the agarose gel, which will be processed downstream (e.g. cloning).

Illuminated with: 4 x 14 W UV bulbs

Exterior dimensions: 13.6 x 11.8 x 3.5 in (34.5 x 30 x 9 cm)

Weight: 5 lbs (2.3 kg)

Power adaptor included

2 year warranty

Accuris cat # E3200.

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Use the Accuris compact UV transilluminator to analyze bands of DNA that have been separated by agarose gel electrophoresis, and stained with ethidium bromide (EtBr), SYBR Green or Accuris SmartGlow, for genotyping purposes, for example.

The scratch-resistant ultraviolet filter glass provides 302 nm UV light (300-312 nm range).  A hinged, UV-protective acrylic lens allows the user to position the lens at an angle and view the illuminated gel safely while allowing access to the gel for band excision.

The small UV transilluminator has only one UV intensity level (i.e. on and off), unlike the larger model, which allows the user to adjust the light intensity with a knob (i.e. variable adjustment).

Illuminated with: 4 x 6 W UV bulbs

Exterior dimensions: 12.8 x 8.5 x 1.9 in (32.5 x 21.5 x 5 cm)

Weight: 2 lbs (0.9 kg)

Power adaptor included

2 year warranty

Accuris cat # E3100.

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Quick-dissolving (5 minutes or less).  No pH adjustment necessary (pH 7.25-7.55).

TBS-T is a buffer used during Western blotting to make blocking buffer (with nonfat dry milk, for example), antibody dilution buffer and wash buffer.

Quicksilver buffers are available for use during nucleic acid electrophoresis, protein electrophoresis, Western blotting, ELISA and cell culture.

1X TBS-T solution contains 137 mM NaCl, 2.7 mM KCl, 50 mM Tris and 0.1% Tween® 20.  Nuclease- and protease-free.

Powdered buffer packs come in a convenient, space-saving dispenser box, 100 pouches/pack.

Makes 100 L of 1 X TBS-T. Equivalent to 10 L of 10X TBS-T.

Accuris catalog # EB1203.

Cost saving equivalent replacement for VWR® TBS-T (Tris Buffered Saline, Tween-20) Buffer Powder Packs (cat # 76371-740).

Accuris TBS-T Buffer Tris Buffered Saline with Tween 20 Powdered Buffer Packs Product Insert.pdf

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Quick-dissolving (5 minutes or less).  No pH adjustment necessary (pH 7.25-7.55).

TBS is a buffer that is used to make blocking buffer during Western blotting and ELISA where proteins are detected using fluorescence (TBS-T isn’t used during the blocking step when detecting proteins by fluorescence because Tween 20 auto-fluoresces). TBS-T can be used in subsequent steps.  TBS is also used during immunohistochemistry.

Quicksilver buffers are available for use during nucleic acid electrophoresis, protein electrophoresis, Western blotting, ELISA and cell culture.

1X TBS solution contains 137 mM NaCl, 2.7 mM KCl and 50 mM Tris.  Nuclease- and protease-free.

Powdered buffer packs come in a convenient, space-saving dispenser box, 100 pouches/pack.

Makes 100 L of 1 X TBS. Equivalent to 10 L of 10X TBS.

Accuris catalog # EB1202.

Cost saving equivalent replacement for VWR® TBS (TRIS Buffered Saline) Buffer Powder Packs (cat # 76371-738).

Accuris TBS Buffer Tris Buffered Saline Powdered Buffer Packs Product Insert.pdf

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Quick-dissolving (5 minutes or less).  No pH adjustment necessary (pH 7.2-7.6).

PBS-T is a buffer used as a wash buffer during Western blotting and ELISA (Enzyme-linked Immunosorbent Assay).

Quicksilver buffers are available for use during nucleic acid electrophoresis, protein electrophoresis, Western blotting, ELISA and cell culture.

1X PBS-T solution contains 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate and 0.05% Tween® 20.  Nuclease- and protease-free.

Powdered buffer packs come in a convenient, space-saving dispenser box, 100 pouches/pack.

Makes 100 L of 1 X PBS-T. Equivalent to 10 L of 10X PBS-T.

Accuris catalog # EB1201.

Cost saving equivalent replacement for VWR® PBS-T (Phosphate Buffered Saline – Tween-20) Buffer Powder Packs (cat # 76371-736).

Accuris PBS-T Buffer Phosphate Buffered Saline with Tween 20 Powdered Buffer Packs Product Insert.pdf

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Quick-dissolving (5 minutes or less).  No pH adjustment necessary (pH 7.2-7.6).

PBS is a buffer that has a variety of uses.  It is used during the manipulation of cells, as it maintains osmotic balance and pH for cells in solution.  It is also used as a blocking buffer (along with nonfat dried milk) during Western blotting and a blocking buffer (along with Bovine Serum Albumin) during ELISA.

Quicksilver buffers are available for use during nucleic acid electrophoresis, protein electrophoresis, Western blotting, ELISA and cell culture.

1X PBS solution contains 137 mM NaCl, 2.7 mM KCl and 10 mM phosphate.  Nuclease- and protease-free.

Powdered buffer packs come in a convenient, space-saving dispenser box, 100 pouches/pack.

Makes 100 L of 1 X PBS. Equivalent to 10 L of 10X PBS.

Accuris catalog # EB1200.

Cost saving equivalent replacement for VWR® PBS (Phosphate Buffered Saline) Buffer Powder Packs (cat # 76371-734).

Accuris PBS Buffer Phosphate Buffered Saline Powdered Buffer Packs Product Insert.pdf

The post PBS Buffer Phosphate Buffered Saline Powdered Buffer Packs appeared first on Morganville Scientific.

Quick-dissolving (5 minutes or less).  No pH adjustment necessary (pH 8.1-8.5).

TGS is used as a running buffer during SDS-PAGE of proteins using Tris-HCl polyacrylamide gels.

Quicksilver buffers are available for use during nucleic acid electrophoresis, protein electrophoresis, Western blotting, ELISA and cell culture.

1X TGS solution contains 25 mM Tris, 192 mM Glycine and 0.1% SDS.  Nuclease- and protease-free.

Powdered buffer packs come in a convenient, space-saving dispenser box, 50 pouches/pack.

Makes 50 L of 1 X TGS. Equivalent to 5 L of 10X TGS.

Accuris catalog # EB1100.

Cost saving equivalent replacement for VWR® TRIS Glycine-SDS Buffer Powder Packs (cat # 76371-728).

Accuris TGS Buffer Tris-Glycine-SDS Powdered Buffer Packs Product Insert.pdf

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Quick-dissolving (5 minutes or less).  No pH adjustment necessary (pH 8.2-8.4).

TAE buffer is ideal for use during agarose gel electrophoresis of DNA.

Quicksilver buffers are available for use during nucleic acid electrophoresis, protein electrophoresis, Western blotting, ELISA and cell culture.

1X TAE solution contains 40 mM Tris, 20 mM acetic acid and 1 mM EDTA.  Nuclease- and protease-free.

Powdered buffer packs come in a convenient, space-saving dispenser box, 100 pouches/pack.

Makes 100 L of 1 X TAE. Equivalent to 2 L of 50X TAE.

Accuris catalog # EB1000.

Cost saving equivalent replacement for VWR® TAE Buffer Powder Packs (cat # 76371-722).

Accuris TAE Buffer Tris-Acetate-EDTA Powdered Buffer Packs Insert.pdf

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DNA extraction and amplification can be performed in same tube (or supernatant containing extracted DNA can be pipetted off of centrifuged lysate).

Ideal for use with mouse tail snips and ear punches.  Also suitable for use with other mammalian fluids and tissues such as blood, buccal cells, hair follicles and Formalin-Fixed Paraffin-Embedded (FFPE) specimens.

Accuris 1 Hour Mammalian Genotyping Kit contains the following components:

• 5X Buffer A (Lysis Buffer)
• 10X Buffer B (Protease Buffer)
• 2X Master Mix (containing Hot Start Taq DNA Polymerase and red dye/DNA loading buffer)

Accuris 1 Hour Mammalian Genotyping Kit is available in the following sizes:

• 8 reactions sample size: 160 uL of 5X Buffer A, 80 uL of 10X Buffer B, 200 uL of 2X Hot Start Taq Master Mix Red (Accuris cat # PR1300-MG-S)
• 80 reactions: 1.6 mL of 5X Buffer A, 800 uL of 10X Buffer B, 2 mL of 2X Hot Start Taq Master Mix Red (Accuris cat # PR1300-MG-80)
• 400 reactions: 8 mL of 5X Buffer A, 4 mL of 10X Buffer B, 10 mL of 2X Hot Start Taq Master Mix Red (Accuris cat # PR1300-MG-400)

Accuris 1 Hour Mammalian Genotyping Kit Protocol.pdf

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Two-step reaction (i.e. Reverse Transcription plus DNA Amplification) is performed in one reaction tube with a single reaction setup.

Amplified DNA is detected by agarose gel electrophoresis; RNA expression is examined by qualitative analysis.

Use as little as 1 pg total RNA (or 0.01 pg mRNA) template.

Accuris One Step RT-PCR Kit contains the following components:

• 2X One-Step Mix (buffer containing Accuris Hot Start Taq Polymerase, 2 mM dNTPs, 6 mM MgCl2 and PCR enhancers)
• 20X Reverse Transcriptase (an RNA-dependant DNA polymerase, derived from MMLV, and an RNase Inhibitor)

Available in 10 (sample size), 50 and 100 reaction sizes (select in dropdown menu).  Reaction sizes are based on total reaction volumes of 50 uL.

Accuris cat # PR1100-S (10 rxn sample size), PR1100-50 (50 rxns), PR1100-100 (100 rxns)

Accuris One Step RT-PCR Kit Protocol.pdf

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