DNA extraction and amplification can be performed in same tube (or supernatant containing extracted DNA can be pipetted off of centrifuged lysate).

Ideal for use with mouse tail snips and ear punches.  Also suitable for use with other mammalian fluids and tissues such as blood, buccal cells, hair follicles and Formalin-Fixed Paraffin-Embedded (FFPE) specimens.

Accuris 1 Hour Mammalian Genotyping Kit contains the following components:

• 5X Buffer A (Lysis Buffer)
• 10X Buffer B (Protease Buffer)
• 2X Master Mix (containing Hot Start Taq DNA Polymerase and red dye/DNA loading buffer)

Accuris 1 Hour Mammalian Genotyping Kit is available in the following sizes:

• 8 reactions sample size: 160 uL of 5X Buffer A, 80 uL of 10X Buffer B, 200 uL of 2X Hot Start Taq Master Mix Red (Accuris cat # PR1300-MG-S)
• 80 reactions: 1.6 mL of 5X Buffer A, 800 uL of 10X Buffer B, 2 mL of 2X Hot Start Taq Master Mix Red (Accuris cat # PR1300-MG-80)
• 400 reactions: 8 mL of 5X Buffer A, 4 mL of 10X Buffer B, 10 mL of 2X Hot Start Taq Master Mix Red (Accuris cat # PR1300-MG-400)

Accuris 1 Hour Mammalian Genotyping Kit Protocol.pdf

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Two-step reaction (i.e. Reverse Transcription plus DNA Amplification) is performed in one reaction tube with a single reaction setup.

Amplified DNA is detected by agarose gel electrophoresis; RNA expression is examined by qualitative analysis.

Use as little as 1 pg total RNA (or 0.01 pg mRNA) template.

Accuris One Step RT-PCR Kit contains the following components:

• 2X One-Step Mix (buffer containing Accuris Hot Start Taq Polymerase, 2 mM dNTPs, 6 mM MgCl2 and PCR enhancers)
• 20X Reverse Transcriptase (an RNA-dependant DNA polymerase, derived from MMLV, and an RNase Inhibitor)

Available in 10 (sample size), 50 and 100 reaction sizes (select in dropdown menu).  Reaction sizes are based on total reaction volumes of 50 uL.

Accuris cat # PR1100-S (10 rxn sample size), PR1100-50 (50 rxns), PR1100-100 (100 rxns)

Accuris One Step RT-PCR Kit Protocol.pdf

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Accuris High Fidelity DNA Polymerase is modified to provide 10X to 100X more accuracy than standard Taq Polymerase.

Accuris High Fidelity DNA Polymerase comes available, has accuracy of, is good for amplification of and leaves amplified DNA ends, as follows:

DNA Polymerase and 5X buffer (containing 15 mM MgCl2, 5 mM dNTPs and PCR enhancers), separated in two tubes:

→Has accuracy of 50X that of unmodified Taq Polymerase

→Good for amplification of more complex sequences, up to 10 kb

→Leaves amplified DNA ends blunt

• 20 units sample size (2 U/uL), 10 uL Hi-Fi DNA Pol + buffer (Accuris cat # PR1000-HF-S)
• 200 units (2 U/uL), 100 uL Hi-Fi DNA Pol + buffer (Accuris cat # PR1000-HF-200)
• 1000 units (2 U/uL), 500 uL Hi-Fi DNA Pol + buffer (Accuris cat # PR1000-HF-1000)

2X Master Mix (containing 6 mM MgCl2, 2 mM dNTPs and PCR enhancers):

→Has accuracy of 100X that of unmodified Taq Polymerase

→Good for amplification of shorter, less complex sequences up to 10 kb

→Leaves amplified DNA ends blunt

• 5 reactions sample size (25 uL total rxn volume), 62.5 uL (Accuris cat # PR1001-HF-S)
• 200 reactions (25 uL total rxn volume), 2.5 mL (Accuris cat # PR1001-HF-200)
• 500 reactions (25 uL total rxn volume), 6.25 mL (Accuris cat # PR1001-HF-500)

2X Hot Start Master Mix (containing 6 mM MgCl2, 2 mM dNTPs and PCR enhancers):

→Has accuracy of 10X that of unmodified Taq Polymerase

→Good for amplification of difficult GC-rich sequences up to 10 kb

→Leaves amplified DNA ends with TA overhangs (i.e. sticky ends)

• 5 reactions sample size (25 uL total rxn volume), 62.5 uL (Accuris cat # PR1001-HFHS-S)
• 200 reactions (25 uL total rxn volume), 2.5 mL (Accuris cat # PR1001-HFHS-200)
• 500 reactions (25 uL total rxn volume), 6.25 mL (Accuris cat # PR1001-HFHS-500)

Accuris High Fidelity DNA Polymerase Protocol.pdf

Accuris High Fidelity DNA Polymerase Master Mix Protocol.pdf

Accuris High Fidelity DNA Polymerase Hot Start Master Mix Protocol.pdf

The post Accuris High Fidelity DNA Polymerase appeared first on Morganville Scientific.

Accuris Hot Start Taq DNA Polymerase has a monoclonal antibody bound to it that prevents its enzymatic activity; this allows the user to set up Polymerase Chain Reactions (PCR) at room temperature without non-specific DNA amplification occurring.

Accuris Hot Start Taq DNA Polymerase has no 3′ to 5′ exonuclease activity (i.e. no proofreading activity).

The monoclonal antibody is denatured (inactivated), and therefore polymerase activated, by running an initial thermocycler incubation for 1-2 minutes at 95°C.

Accuris Hot Start Taq DNA Polymerase comes available as:

Taq Pol and 5X buffer (containing 15 mM MgCl2 and PCR enhancers), separated in two tubes:

• 50 units sample size (5 U/uL), 10 uL Taq + buffer (Accuris cat # PR1000-HS-S)
• 500 units (5 U/uL), 100 uL Taq + buffer (Accuris cat # PR1000-HS-500)
• 1000 units (5 U/uL), 200 uL Taq + buffer (Accuris cat # PR1000-HS-1000)
• 6000 units (5 U/uL), 1200 uL Taq + buffer (Accuris cat # PR1000-HS-6000)

2X Master Mix (containing 6 mM MgCl2, 2 mM dNTPs and PCR enhancers):

• 5 reactions sample size (50 uL total rxn volume) / 10 reactions (25 uL total rxn volume), 125 uL (Accuris cat # PR1001-HS-S)
• 200 reactions (50 uL total rxn volume) / 400 reactions (25 uL total rxn volume), 5 mL (Accuris cat # PR1001-HS-200)
• 1000 reactions (50 uL total rxn volume) / 2000 reactions (25 uL total rxn volume), 25 mL (Accuris cat # PR1001-HS-1000)

2X Master Mix with red dye/DNA loading buffer (containing 6 mM MgCl2, 2 mM dNTPs, PCR enhancers and red dye; red dye DNA loading buffer visually aids the user during pipetting; density of buffer allows DNA sample to sink to the bottom of the agarose gel well):

• 5 reactions sample size (50 uL total rxn volume) / 10 reactions (25 uL total rxn volume), 125 uL (Accuris cat # PR1001-HSR-S)
• 200 reactions (50 uL total rxn volume) / 400 reactions (25 uL total rxn volume), 5 mL (Accuris cat # PR1001-HSR-200)
• 1000 reactions (50 uL total rxn volume) / 2000 reactions (25 uL total rxn volume), 25 mL (Accuris cat # PR1001-HSR-1000)

Accuris Hot Start Taq Polymerase Protocol.pdf

Accuris Hot Start Taq Polymerase Master Mix Protocol.pdf

The post Accuris Hot Start Taq Polymerase appeared first on Morganville Scientific.

Accuris standard Taq DNA Polymerase is not Hot Start (i.e. enzymatic activity is not blocked by a monoclonal antibody) and has no 3′ to 5′ exonuclease activity (i.e. no proofreading activity).

Accuris standard Taq DNA Polymerase comes available as:

Taq Pol and 5X buffer (containing 15 mM MgCl2 and PCR enhancers), separated in two tubes:

• 50 units sample size (5 U/uL), 10 uL Taq + buffer (Accuris cat # PR1000-S)
• 500 units (5 U/uL), 100 uL Taq + buffer (Accuris cat # PR1000-500)
• 1000 units (5 U/uL), 200 uL Taq + buffer (Accuris cat # PR1000-1000)
• 6000 units (5 U/uL), 1200 uL Taq + buffer (Accuris cat # PR1000-6000)

2X Master Mix (containing 6 mM MgCl2, 2 mM dNTPs and PCR enhancers):

• 5 reactions sample size (50 uL total rxn volume) / 10 reactions (25 uL total rxn volume), 125 uL (Accuris cat # PR1001-S)
• 200 reactions (50 uL total rxn volume) / 400 reactions (25 uL total rxn volume), 5 mL (Accuris cat # PR1001-200)
• 1000 reactions (50 uL total rxn volume) / 2000 reactions (25 uL total rxn volume), 25 mL (Accuris cat # PR1001-1000)

2X Master Mix with red dye/DNA loading buffer (containing 6 mM MgCl2, 2 mM dNTPs, PCR enhancers and red dye; red dye DNA loading buffer visually aids the user during pipetting; density of buffer allows DNA sample to sink to the bottom of the agarose gel well):

• 5 reactions sample size (50 uL total rxn volume) / 10 reactions (25 uL total rxn volume), 125 uL (Accuris cat # PR1001-R-S)
• 200 reactions (50 uL total rxn volume) / 400 reactions (25 uL total rxn volume), 5 mL (Accuris cat # PR1001-R-200)
• 1000 reactions (50 uL total rxn volume) / 2000 reactions (25 uL total rxn volume), 25 mL (Accuris cat # PR1001-R-1000)

Accuris Taq Polymerase Protocol.pdf

Accuris Taq Polymerase Master Mix Protocol.pdf

The post Accuris Taq Polymerase appeared first on Morganville Scientific.

dNTPs are added by DNA Polymerase, during the PCR, to the growing strands of DNA that are being copied (synthesized).

Accuris dNTPs are available individually as a set of four separate nucleotides—dATP (deoxyadenosine triphosphate), dTTP (deoxythymidine triphosphate), dCTP (deoxycytidine triphosphate) and dGTP (deoxyguanosine triphosphate)—or as a ready-to-use mix of all four nucleotides (click here for the mix).

The dNTP set contains 100 mM concentration (25 umol/250 uL or 100 umol/1000 uL) of each nucleotide in each tube.  The set is available in volumes of 250 uL x 4 dNTPs and 1 mL x 4 dNTPs.

Accuris dNTPs are purified to greater than 99% by means of HPLC.  They are free of PCR inhibitors such as proteases.

Accuris catalog # PR3101-S-1 (250 uL x 4), PR3101-S-4 (1 mL x 4).

Accuris 100 mM dNTP Set Product Insert.pdf

The post Accuris 100 mM dNTP Set appeared first on Morganville Scientific.

dNTPs are added by DNA Polymerase, during the PCR, to the growing strands of DNA that are being copied (synthesized).

Accuris dNTPs are available as a ready-to-use mix of all four nucleotides—dATP (deoxyadenosine triphosphate), dTTP (deoxythymidine triphosphate), dCTP (deoxycytidine triphosphate) and dGTP (deoxyguanosine triphosphate)—or individually as a set of four separate nucleotides (click here for the set).

The dNTP mix contains 10 mM concentration (5 umol/500 uL or 20 umol/2000 uL) of each nucleotide in one tube, 40 mM total concentration of the four dNTPs.  The mix is available in 0.5 mL and 2.0 mL volumes.

Accuris dNTPs are purified to greater than 99% by means of HPLC.  They are free of PCR inhibitors such as proteases.

Accuris catalog # PR3040-M-1 (0.5 mL), PR3040-M-4 (2.0 mL).

Accuris 40 mM dNTP Mix Product Insert.pdf

The post Accuris 40 mM dNTP Mix appeared first on Morganville Scientific.

Accuris 1 kb DNA Ladder contains 13 DNA bands ranging from 250 bp to 10 kb.

Accuris SmartCheck 1 kb DNA Ladder comes 500 uL/each; that’s enough ladder for 100 DNA agarose gel electrophoresis lanes.

Purchase 5 x 500 uL for a significant additional discount (select in dropdown menu).

Contains loading buffer and the tracking dyes bromophenol blue (purple color, runs at about 500 bp) and xylene cyanol (blue in color, runs at about 4 kb).

Recommended load is 5 uL per gel lane. Concentration is 0.1 mg/mL.

Buffer is composed of 10 mM Tris-HCl (pH 8.0), 5 mM EDTA, 12.5% glycerol, 0.008% bromophenol blue and 0.008% xylene cyanol.

Accuris DNA ladders ship at room temperature; store them long term at -20°C (for up to 15 months), and short term at 4°C (for up to 6 months).

Accuris catalog # PR4100-100 (500 uL), PR4100-500 (500 uL x 5).

Accuris SmartCheck 1 kb DNA Ladder Protocol.pdf

The post Accuris SmartCheck 1 kb DNA Ladder appeared first on Morganville Scientific.

Accuris 50 bp DNA Ladder contains 13 DNA bands ranging from 50 bp to 1.5 kb.

Accuris SmartCheck 50 bp DNA Ladder comes 500 uL/each; that’s enough ladder for 100 DNA agarose gel electrophoresis lanes.

Purchase 5 x 500 uL for a significant additional discount (select in dropdown menu).

Contains loading buffer and the tracking dyes bromophenol blue (purple color, runs at about 500 bp) and xylene cyanol (blue in color, runs at about 4 kb).

Recommended load is 5 uL per gel lane. Concentration is 0.1 mg/mL.

Buffer is composed of 10 mM Tris-HCl (pH 8.0), 5 mM EDTA, 12.5% glycerol, 0.008% bromophenol blue and 0.008% xylene cyanol.

Accuris DNA ladders ship at room temperature; store them long term at -20°C (for up to 15 months), and short term at 4°C (for up to 6 months).

Accuris catalog # PR4005-100 (500 uL), PR4005-500 (500 uL x 5).

Accuris SmartCheck 50 bp DNA Ladder Protocol.pdf

The post Accuris SmartCheck 50 bp DNA Ladder appeared first on Morganville Scientific.

Accuris 100 bp DNA Ladder contains 10 DNA bands ranging from 100 bp to 1 kb.

Accuris SmartCheck 100 bp DNA Ladder comes 500 uL/each; that’s enough ladder for 100 DNA agarose gel electrophoresis lanes.

Purchase 5 x 500 uL for a significant additional discount (select in dropdown menu).

Contains loading buffer and the tracking dyes bromophenol blue (purple color, runs at about 500 bp) and xylene cyanol (blue in color, runs at about 4 kb).

Recommended load is 5 uL per gel lane. Concentration is 0.1 mg/mL.

Buffer is composed of 10 mM Tris-HCl (pH 8.0), 5 mM EDTA, 12.5% glycerol, 0.008% bromophenol blue and 0.008% xylene cyanol.

Accuris DNA ladders ship at room temperature; store them long term at -20°C (for up to 15 months), and short term at 4°C (for up to 6 months).

Accuris catalog # PR4010-100 (500 uL), PR4010-500 (500 uL x 5).

Accuris SmartCheck 100 bp DNA Ladder Protocol.pdf

The post Accuris SmartCheck 100 bp DNA Ladder appeared first on Morganville Scientific.