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6X SDS Protein Loading Buffer for SDS-PAGE.
Morganville Scientific 6X SDS Protein Loading Buffer (Laemmli buffer) is used for the preparation of protein samples for SDS-polyacrylamide gel electrophoresis (SDS-PAGE). After the addition of the reducing agent ß-mercaptoethanol (or DTT), the protein loading buffer will contain all of the necessary components for complete disruption of high-order protein structures.
6X Protein Loading Buffer is ideal because the protein sample prepared in 6X buffer will be more concentrated than protein sample prepared in 4X or 2X buffer (i.e. more protein and less loading buffer per well).
6X Laemmli SDS PAGE Sample Loading Buffer, 25 mL.
375 mM Tris.HCl
0.03% Bromophenol blue
Contents: 25 mL
Storage: Ambient Temperature
Note: ß-mercaptoethanol rapidly oxidizes in protein loading buffer. Fresh 6X protein loading buffer should be prepared every time.
Excellent alternative to Biorad 2X Laemmli sample buffer (catalog # 161-0737).