SmartGlow Safe Nucleic Acid Stain, Green Stain for DNA and RNA.
Accuris SmartGlow safe nucleic acid stain for use during agarose or polyacrylamide gel electrophoresis (PAGE) of DNA or RNA. Repaces hazardous ethidium bromide (EtBr), which is mutagenic. More sensitive nucleic acid detection than ethidium bromide (as little as 0.1 ng).
Two types of SmartGlow available (select in dropdown menu):
SmartGlow PS, 1 mL, is a pre-stain that is added to the agarose or polyacrylamide solution (5 uL/100 mL), and then the gel is poured as normal. It may also be added to the running buffer (5 uL/100 mL) in addition to the gel solution for enhanced results.
SmartGlow LD, 1 mL, is a 6X loading dye that is added directly to the samples (1 uL SmartGlow LD/5 uL sample), with no need to add it to the gel or running buffer. Samples are then run as normal.
Electrophorese samples and examine them with the UV transilluminator. SmartGlow PS glows green when bound to DNA and glows red when bound to RNA. SmartGlow LD glows green when bound to DNA and RNA.
Excellent, safer replacement for BioRad Ethidium Bromide solution, 10 mg/mL (Cat # 161-0433).
Cost saving equivalent replacement for Invitrogen™ SYBR™ Safe™ DNA Gel Stain (Cat # S33102).
Accuris Catalog # E4500-PS (SmartGlow PS), E4500-LD (SmartGlow LD).