6X SDS Protein Loading Buffer for SDS-PAGE.
Morganville Scientific 6X SDS Protein Loading Buffer (Laemmli buffer) is used for the preparation of protein samples for SDS-polyacrylamide gel electrophoresis (SDS-PAGE). After the addition of the reducing agent ß-mercaptoethanol (or DTT), the protein loading buffer will contain all of the necessary components for complete disruption of high-order protein structures.
6X Protein Loading Buffer is ideal because the protein sample prepared in 6X buffer will be more concentrated than protein sample prepared in 4X or 2X buffer (i.e. more protein and less loading buffer per well).
6X Laemmli SDS PAGE Sample Loading Buffer, 25 mL.
375 mM Tris.HCl, pH 6.8
0.03% Bromophenol blue
Contents: 25 mL
Storage: Ambient Temperature
Note: ß-mercaptoethanol rapidly oxidizes in protein loading buffer. Fresh 6X protein loading buffer should be prepared every time.
Excellent alternative to Biorad 2X Laemmli sample buffer (catalog # 161-0737).
For research purposes only. Not intended for human or animal therapeutic or diagnostic uses.
Research publications that include Morganville Scientific 6X SDS Protein Loading Buffer (cat # LB0100) in the Materials and Methods section:
Larisa Gofman, Nicole C. Fernandes, Raghava Potula; Relative Role of Akt, ERK and CREB in Alcohol-Induced Microglia P2X4R Receptor Expression. Alcohol 2016; 51 (6): 647-654. doi: 10.1093/alcalc/agw009. [LINK TO ARTICLE, NCBI]
Kane JR, Stanley DJ, Hultquist JF, et al. Lineage-Specific Viral Hijacking of Non-Canonical E3 Ubiquitin Ligase Cofactors in the Evolution of Vif Anti-APOBEC3 Activity. Cell reports. 2015;11(8):1236-1250. doi:10.1016/j.celrep.2015.04.038. [LINK TO ARTICLE, NCBI, found in supplemental information]
P Blackwood M.D. B, R Wood B.S. D, Y Yuan B.S. C, et al. Urinary Claudin-2 Measurements as a Predictor of Necrotizing Enterocolitis: A Pilot Study. Journal of Neonatal Surgery. 2015;4(4):43. [LINK TO ARTICLE, NCBI]
Woodard CM, Campos BA, Kuo S-H, et al. iPS Cell-Derived Dopamine Neurons Reveal Differences between Monozygotic Twins Discordant for Parkinson’s Disease. Cell reports. 2014;9(4):1173-1182. doi:10.1016/j.celrep.2014.10.023. [LINK TO ARTICLE, NCBI]