Accuris High Fidelity DNA Polymerase

$5.61$480.59

Accuris High Fidelity DNA Polymerase, modified with enhanced proofreading activity. Comes available as enzyme + buffer, separated, or in 2X Master Mix form (with or without Hot Start feature).

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SKU: TAQ0300 Categories: ,

Description

Accuris High Fidelity DNA Polymerase is a modified Pfu DNA polymerase with proofreading activity (i.e. 3′ to 5′ exonuclease activity).  It is ideal when highly accurate amplification of long DNA sequences is required, such as when cloning.

Accuris High Fidelity DNA Polymerase is modified to provide 10X to 100X more accuracy than standard Taq Polymerase.

Accuris High Fidelity DNA Polymerase comes available, has accuracy of, is good for amplification of and leaves amplified DNA ends, as follows:

DNA Polymerase and 5X buffer (containing 15 mM MgCl2, 5 mM dNTPs and PCR enhancers), separated in two tubes:

→Has accuracy of 50X that of unmodified Taq Polymerase

→Good for amplification of more complex sequences, up to 10 kb

→Leaves amplified DNA ends blunt

  • 20 units sample size (2 U/uL), 10 uL Hi-Fi DNA Pol + buffer (Accuris cat # PR1000-HF-S)
  • 200 units (2 U/uL), 100 uL Hi-Fi DNA Pol + buffer (Accuris cat # PR1000-HF-200)
  • 1000 units (2 U/uL), 500 uL Hi-Fi DNA Pol + buffer (Accuris cat # PR1000-HF-1000)

2X Master Mix (containing 6 mM MgCl2, 2 mM dNTPs and PCR enhancers):

→Has accuracy of 100X that of unmodified Taq Polymerase

→Good for amplification of shorter, less complex sequences up to 10 kb

→Leaves amplified DNA ends blunt

  • 5 reactions sample size (25 uL total rxn volume), 62.5 uL (Accuris cat # PR1001-HF-S)
  • 200 reactions (25 uL total rxn volume), 2.5 mL (Accuris cat # PR1001-HF-200)
  • 500 reactions (25 uL total rxn volume), 6.25 mL (Accuris cat # PR1001-HF-500)

2X Hot Start Master Mix (containing 6 mM MgCl2, 2 mM dNTPs and PCR enhancers):

→Has accuracy of 10X that of unmodified Taq Polymerase

→Good for amplification of difficult GC-rich sequences up to 10 kb

→Leaves amplified DNA ends with TA overhangs (i.e. sticky ends)

  • 5 reactions sample size (25 uL total rxn volume), 62.5 uL (Accuris cat # PR1001-HFHS-S)
  • 200 reactions (25 uL total rxn volume), 2.5 mL (Accuris cat # PR1001-HFHS-200)
  • 500 reactions (25 uL total rxn volume), 6.25 mL (Accuris cat # PR1001-HFHS-500)

Accuris High Fidelity DNA Polymerase Protocol.pdf

Accuris High Fidelity DNA Polymerase Master Mix Protocol.pdf

Accuris High Fidelity DNA Polymerase Hot Start Master Mix Protocol.pdf

Additional information

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DNA Polymerase and 5X buffer, 2X Master Mix, 2X Hot Start Master Mix

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20 units (sample size), 200 units, 1000 units, 5 reactions (sample size), 200 reactions, 500 reactions