Accuris Taq Polymerase

$5.61$677.88

Accuris standard Taq DNA Polymerase (non-hot start, no proofreading activity). Comes available as enzyme + buffer, separated, or in Master Mix form (with or without red DNA loading buffer).

Compare
SKU: TAQ0100 Categories: ,

Description

Accuris Taq Polymerase is a basic DNA polymerase with good solubility and DNA affinity, for use during standard PCR such as when genotyping mice.

Accuris standard Taq DNA Polymerase is not Hot Start (i.e. enzymatic activity is not blocked by a monoclonal antibody) and has no 3′ to 5′ exonuclease activity (i.e. no proofreading activity).

Accuris standard Taq DNA Polymerase comes available as:

Taq Pol and 5X buffer (containing 15 mM MgCl2 and PCR enhancers), separated in two tubes:

  • 50 units sample size (5 U/uL), 10 uL Taq + buffer (Accuris cat # PR1000-S)
  • 500 units (5 U/uL), 100 uL Taq + buffer (Accuris cat # PR1000-500)
  • 1000 units (5 U/uL), 200 uL Taq + buffer (Accuris cat # PR1000-1000)
  • 6000 units (5 U/uL), 1200 uL Taq + buffer (Accuris cat # PR1000-6000)

2X Master Mix (containing 6 mM MgCl2, 2 mM dNTPs and PCR enhancers):

  • 5 reactions sample size (50 uL total rxn volume) / 10 reactions (25 uL total rxn volume), 125 uL (Accuris cat # PR1001-S)
  • 200 reactions (50 uL total rxn volume) / 400 reactions (25 uL total rxn volume), 5 mL (Accuris cat # PR1001-200)
  • 1000 reactions (50 uL total rxn volume) / 2000 reactions (25 uL total rxn volume), 25 mL (Accuris cat # PR1001-1000)

2X Master Mix with red dye/DNA loading buffer (containing 6 mM MgCl2, 2 mM dNTPs, PCR enhancers and red dye; red dye DNA loading buffer visually aids the user during pipetting; density of buffer allows DNA sample to sink to the bottom of the agarose gel well):

  • 5 reactions sample size (50 uL total rxn volume) / 10 reactions (25 uL total rxn volume), 125 uL (Accuris cat # PR1001-R-S)
  • 200 reactions (50 uL total rxn volume) / 400 reactions (25 uL total rxn volume), 5 mL (Accuris cat # PR1001-R-200)
  • 1000 reactions (50 uL total rxn volume) / 2000 reactions (25 uL total rxn volume), 25 mL (Accuris cat # PR1001-R-1000)

Accuris Taq Polymerase Protocol.pdf

Accuris Taq Polymerase Master Mix Protocol.pdf

Additional information

Weight N/A
Dimensions N/A
Select

Taq Pol and 5X buffer, 2X Master Mix, 2X Master Mix with red dye/DNA loading buffer

Select Volume

50 units (sample size), 500 units, 1000 units, 6000 units, 5 reactions (sample size), 200 reactions, 1000 reactions