qMax cDNA Synthesis Kit

$9.35$811.58

Accuris qMax cDNA Synthesis Kit & qMax 1st Strand cDNA Synthesis Flex Kit: High yields of cDNA from low quantities of total RNA or mRNA. Available with primers in the buffer (original kit) or separated for assay design flexibility (flex kit).

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SKU: RTQ0600 Categories: ,

Description

Accuris qMax cDNA Synthesis Kit and qMax 1st Strand cDNA Synthesis Flex Kit allow the user to generate high yields of cDNA from low amounts of RNA starting template (total RNA or purified mRNA), via reverse transcription.  cDNA is then used for further experimentation and analysis (e.g. Quantitative PCR).


The qMax™ cDNA Synthesis Kit contains two components:

  • An optimized 5X buffer that contains anchored oligo (dT) primers, random hexamers, dNTPs, 20mM MgCl, stabilizers and enhancers
  • 20X Reverse Transcriptase (an RNA-dependant DNA polymerase, derived from MMLV, and an RNase Inhibitor)

→Works optimally with 4 pg to 0.5 ug of total RNA or oligo(dT) purified mRNA

The qMax™ 1st Strand cDNA Synthesis Flex Kit contains four components:

  • An optimized 5X buffer that contains dNTPs, 20mM MgCl, stabilizers and enhancers
  • 20X Reverse Transcriptase (an RNA-dependant DNA polymerase, derived from MMLV, and an RNase Inhibitor)
  • Solution of oligo (dT) primers
  • Solution of random hexamer primers

→Works optimally with 10 pg to 2.0 ug of total RNA or 5 pg to 0.5 ug of oligo (dT) purified mRNA


→qMax Reverse Transcriptase is quite thermostable and will allow for reverse transcription at a higher temperature (45°C), which helps alleviate problems caused by RNA secondary structure formation.
→Anchored oligo (dT) primers will bind to the very 3′ end of the mRNA, preventing the amplification of truncated cDNAs by not binding to internal poly(A) sites.
→Separate solutions of oligo (dT) primers and random hexamer primers gives the user more flexibility in protocol design.

qMax cDNA Synthesis Kit is available in 5 (sample size), 25100 and 250 reactions sizes (select in dropdown menu).

qMax 1st Strand cDNA Synthesis Flex Kit is available in 5 (sample size), 50 and 200 reactions sizes (select in dropdown menu).

Reaction sizes are based on total reaction volumes of 20 uL.


Accuris catalog numbers:

qMax cDNA Synthesis Kit: 5 rxns (PR2100-C-S), 25 rxns (PR2100-C-25), 100 rxns (PR2100-C-100), 250 rxns (PR2100-C-250)

qMax 1st Strand cDNA Synthesis Flex Kit: 5 rxns (PR2110-S), 50 rxns (PR2110-50), 200 rxns (PR2110-200)


Accuris qMax cDNA Synthesis Kit Protocol.pdf

Accuris qMax 1st Strand cDNA Synthesis Flex Kit Protocol.pdf

Additional information

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Select Kit

qMax cDNA Synthesis Kit, qMax 1st Strand cDNA Synthesis Flex Kit

Select Reaction Quantity

5 reactions (sample size), 25 reactions, 50 reactions, 100 reactions, 200 reactions, 250 reactions